Localization Precision in Stepwise Photobleaching Experiments
نویسندگان
چکیده
منابع مشابه
Localization precision in stepwise photobleaching experiments.
The precise determination of the position of fluorescent labels is essential for the quantitative study of biomolecular structures by various localization microscopy techniques. Localization by stepwise photobleaching is especially suited for measuring nanometer-scale distances between two labels; however, the precision of this method has remained elusive. Here, we show that shot noise from oth...
متن کاملChallenges and artifacts in quantitative photobleaching experiments.
Confocal fluorescence recovery after photobleaching (FRAP) is today the prevalent tool when studying the diffusional and kinetic properties of proteins in living cells. Obtaining quantitative data for diffusion coefficients via FRAP, however, is challenged by the fact that both bleaching and scanning take a finite time. Starting from an experimental case, it is shown by means of computer simula...
متن کاملMicroRNA maturation: stepwise processing and subcellular localization.
MicroRNAs (miRNAs) constitute a novel, phylogenetically extensive family of small RNAs ( approximately 22 nucleotides) with potential roles in gene regulation. Apart from the finding that miRNAs are produced by Dicer from the precursors of approximately 70 nucleotides (pre-miRNAs), little is known about miRNA biogenesis. Some miRNA genes have been found in close conjunction, suggesting that the...
متن کاملSupersymmetry versus Precision Experiments Revisited
We study constraints on the Minimal Supersymmetric Standard Model from electroweak experiments. We find that the light sfermions always make the fit worse than the Standard Model, while the light chargino generally make the fit slightly better through the oblique corrections. The best overall fit to the precision measurements are found when the mass of lighter chargino is about 100 GeV and the ...
متن کاملPrecision Electroweak Experiments at Lep
During the operation of the LEP e + e ? collider at the Z resonance from 1989 to 1995 the four experiments ALEPH, DELPHI, L3 and OPAL have collected about 16 million decays of the Z into hadrons and charged leptons. The precise measurements of the total cross section allow an accurate determination of the properties of the Z. Including data up to 1994 one obtains for the mass, the total and par...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.09.035